Optimization of CFTR-mRNA transfection in human nasal epithelial cells

Optimization of CFTR-mRNA transfection in human nasal epithelial cells

Background: Cystic fibrosis (CF) is the most common life-threatening inherited disease in the Caucasian population. It is caused by genetic defects in the cystic fibrosis transmembrane conductance regulator gene (CFTR), a cAMP regulated chloride-bicarbonate channel mainly located in the apical membrane of polarized epithelial cells. CFTR is proposed to regulate other proteins, including the epi...

CFTR Expression Analysis in Human Nasal Epithelial Cells by Flow Cytometry

RATIONALE Unbiased approaches that study aberrant protein expression in primary airway epithelial cells at single cell level may profoundly improve diagnosis and understanding of airway diseases. We here present a flow cytometric procedure to study CFTR expression in human primary nasal epithelial cells from patients with Cystic Fibrosis (CF). Our novel approach may be important in monitoring o...

EXPRESSION OF CFTR mRNA IN NASAL EPITHELIUM AND VAS DEFERENS

mRNA-transfection of Retinal Pigmented Epithelial cells 1 Efficient delivery and functional expression of transfected modified mRNA in human Embryonic Stem Cell-derived Retinal Pigmented Epithelial cells

Geneand cell-based therapies are promising strategies for the treatment of degenerative retinal diseases such as age-related macular degeneration (AMD), Stargardt disease and Retinitis Pigmentosa. Cellular engineering before transplantation may allow the delivery of cellular factors that can promote functional improvements such as increased engraftment or survival of transplanted cells. A curre...

Optimization of SW480 Colon Cancer Cells Transfection with Lipofectamine 2000

ABSTRACT           Background and Objectives: Nonviral carriers including those based on synthetic cationic lipids, offer several advantages over the viral counterparts. These carriers are able to form complexes with nucleic acids and deliver genes into the cells via the cellular endocytosis pathway, without significant toxicity. The level of transg...